Author  
Place of duty  
Title   Salicylate 에 의한 A549 세포의 Apoptosis ( Salicylate Induced Apoptosis in A549 Cells )
Publicationinfo   2000 Jan; 032(04): 734-742.
Key_word   Salicylate, Lung neoplasm, Apoptosis
Full-Text  
Abstract   Purpose: Nonsteroidal antiinflammatory drugs (NSAIDs) have been shown to exist chemopreventive activity against colon cancers. In this study, we examined whether salicylate affects the survival of A549 cells, and investigated the presence of apoptosis. Materials and Methods: We used A549 human lung cancer cell line. The measurement of cytotoxic concentration of salicylate was performed by MTT assay method. In order to test the involvement of apoptosis, we performed TUNEL assay, DAPI staining, flow cytometric analysis and RT-PCR. Results: We showed that salicylate can potently induce apoptosis in A549 cells. A549 cells under- went apoptosis in treatment with salicylate at pharmacological concentration (5 mM). Conclusion: Herein, our data provide a potential mechanism for chemopreventive activity of salicylate and suggest that salicylate may have therapeutic potential for the treatment of lung cancer.
저 자   박연희(Yeon Hee Park),설재일(Jae Il Seol),김현일(Hyun Il Kim),김미자(Mi Ja Kim),이희제(Hee Jae Lee),김순애(Soon Ae Kim),김창주(Chang Ju Kim),정주호(Joo Ho Chung)